Principle

The system uses reagents quickly permeating the biological samples and thus simplifies total RNA extraction in combination of the stringency of guanidine-thiocyanate lysis and silica-gel-membrane purification. This system has no need of phenol/chloroform extraction and centrifugation with CsCl gradients or precipitation with LiCl or ethanol.

Downstream Applications

RNA purified by Viogene Total RNA Extraction System has A₂₆₀/A₂₈₀ ratio of 1.9~2.1, and is ideal for all applications including:

• Northern, dot and slot blotting
• RT-PCR / Quantitative real-time PCR
• Poly A⁺ RNA selection
• cDNA Synthesis/ Primer extension
• Array analysis
• In vitro translation