VIOGENE- Genomic DNA Extraction

Blood and Tissue Genomic DNA Miniprep System

Cat No.: GG1002
Package: 250 preps
List Price: $618.20

  • Rapid isolation of whole blood, tissue, cells genomic DNA
  • Simple spin column method
  • No organic solvent extraction (without phenol/ chloroform) & no ethanol precipitation
  • Free of contaminants and enzyme inhibitors
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Product Information

Principle

Blood and Tissue Genomic DNA Miniprep System uses the silica-gel membrane technology for simple and fast isolation of genomic DNA without phenol/chloroform or ethanol precipitation. Sample homogenization is not necessary since tissues are directly lysed by Proteinase K. The buffer system is optimized to allow selective binding of genomic DNA to the silica-gel membrane. A simple centrifugation protocol effectively removes contaminants such as proteins, divalent cations, and secondary metabolites. Pure genomic DNA is then eluted in water or low-salt buffer, ready to use. Special protocols and guidelines are provided for different samples, such as tissues, culture cells, blood, mouse tails, bacteria and yeast. Purified DNA is free from contaminants and enzyme inhibitors, and typically has A260/A280 ratios between 1.7 and 1.9. The genomic DNA is sized up to 50-kb, with predominant fragments of 30-kb.

Downstream Applications

  • Restriction digestion
  • Southern blotting
  • RAPD
  • RFLP
  • PCR, Real-time PCR
  • PCR and qPCR
  • NGS
  • Genome editing

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