Principle

Blood and Tissue Genomic DNA Miniprep System uses the silica-gel membrane technology for simple and fast isolation of genomic DNA without phenol/chloroform or ethanol precipitation. Sample homogenization is not necessary since tissues are directly lysed by Proteinase K. The buffer system is optimized to allow selective binding of genomic DNA to the silica-gel membrane. A simple centrifugation protocol effectively removes contaminants such as proteins, divalent cations, and secondary metabolites. Pure genomic DNA is then eluted in water or low-salt buffer, ready to use. Special protocols and guidelines are provided for different samples, such as tissues, culture cells, blood, mouse tails, bacteria and yeast. Purified DNA is free from contaminants and enzyme inhibitors, and typically has A_260/A₂₈₀ ratios between 1.7 and 1.9. The genomic DNA is sized up to 50-kb, with predominant fragments of 30-kb.

Downstream Applications

• Restriction digestion
• Southern blotting
• RAPD
• RFLP
• PCR, Real-time PCR
• PCR and qPCR
• NGS
• Genome editing