0.1 to 10 ml Serum or Plasma cfDNA Extraction Kit provides a simple and reliable method for the rapid preparation of high-quality circulating cell-free DNA from serum or plasma. A combination of vacuum and spin method efficiently recover total cfDNA from 100 μl up to 10 ml of serum or plasma sample. The MM Vacuum-Spin column technology allows for ultra-pure DNA to be eluted in as little as 30 μl of Elution Buffer or water, the resulting DNA is suitable for all subsequent analyses and molecular manipulations such as qPCR, dPCR, Next-generation sequencing, and biomarkers discovery. Yield of 10–30 ng of cfDNA per ml of serum or plasma is typical. The majority of cfDNA fragments are approximately 150-170 bp.