Principle

Viogene Low-Endotoxin Plasmid DNA Maxiprep Extraction System provides a simple, fast and cost-effective method to purify plasmid DNA without phenol/chloroform extraction. It is based on binding of DNA to silica-based membranes in chaotropic salts. An average yield of 500 mg of plasmid DNA can be expected from 100 ml overnight bacterial culture.

Downstream Applications

• Restriction digestion
• Radioactive and fluorescent sequencing
• Transformation / Transfection
• Ligation
• PCR, RAPD
• NGS