Principle

The Viogene Plant Genomic DNA Extraction System uses silica-gel membrane technology for simple and fast isolation of plant genomic DNA without phenol/chloroform or ethanol precipitation. Genomic DNA purified by the system is free from contaminants and enzyme inhibitors, and typically has A₂₆₀ / A₂₈₀ ratios between 1.7 and 1.9. The DNA is sized up to 50Kb, with predominant fragments of 30Kb.

Downstream Applications

• Restriction digestion
• Southern blotting
• RAPD, RFLP
• PCR, qPCR
• NGS
• Genome editing