Viogene eDNA Water is designed for the isolation of eDNA from 20 ml of environmental water samples such as river, stream, creek, lake, pond, lagoon, bay, sea or ocean. The eDNA Water recovers small to large eDNA fragments. A simple one step gravity filter-column protocol without arduous vacuum or pressure filter steps. DNA yield strongly depends on the individual sample type and processed water volume. Total DNA yields of a few ng up to 2 µg have been observed from 20 ml of environmental water. Isolated DNA is suitable for diverse downstream applications such as PCR, qPCR and metabarcoding.