Principle

Viogene Low-Endotoxin Plasmid DNA Miniprep Extraction System provides a simple, fast and cost-effective method to purify plasmid DNA without phenol/chloroform extraction. It is based on binding of DNA to silica-based membranes in chaotropic salts. An average yield of 1 to 40 mg of plasmid DNA can be expected from 1 to 5 ml overnight bacterial culture.

Downstream Applications

Viogene Low-Endotoxin Plasmid DNA Miniprep Extraction System provides reproducible yields of high-purity DNA suitable for using in most applications, including:

• Transfection
• Transformation
• Ligation and cloning
• Manual or automated sequencing, including radioactive and fluorescent sequencing
• PCR and qPCR applications
• NGS