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Plant Total RNA Extraction Miniprep System
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Product
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Cat. No
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Package
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Contents
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MSDS
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Catalog
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Protocol
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Plant Total RNA Extraction Miniprep System |
GPR1001 |
50preps |
50 Plant Total RNA Mini Columns,
100 Collection Tubes,
50 Shearing Tubes,
50 Elution Tubes,
Buffers, Protocol,
RNase-free ddH2O |
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| GPR1002 |
250preps |
250 Plant Total RNA Mini Columns,
500 Collection Tubes,
250 Shearing Tubes,
Buffers, Protocol,
RNase-free ddH2O |
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Principle
The system uses some reagents quickly permeating the biological samples and simplifies total RNA isolation by combination of the stringency of guanidine-thiocyanate lysis and silica-gel-membrane purification. This system has no need for phenol/ chloroform extraction and centrifugation with CsCl gradients or precipitation with LiCl or ethanol. Plant tissues that contain sticky secondary metabolites are lysed by additional provided PRX Buffer.
Downstream Applications
RNA purified by Viogene® Plant Total RNA Extraction System has A260/A280 ratio of 1.9~2.1 and is ideal for all applications, including:
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Northern blotting
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RT-PCR
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cDNA Synthesis
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In vitro translation
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